Anti-NBN monoclonal antibody, clone OCT2-612

Antibody Isotype

IgG1

Clone

OCT2-612

Species Reactivity

Human

Immunogen

Synthetic peptide: GSKNVDLSGRQERKQ, corresponding to amino acids 206-220 of Human p95 NBS1

Conjugate

Alternative Names

NBN; nibrin; NBS, NBS1, Nijmegen breakage syndrome 1 (nibrin); AT V1; AT V2; ATV; cell cycle regulatory protein p95; Nijmegen breakage syndrome 1 (nibrin); p95 protein of the MRE11/RAD50 complex; NBS; P95; NBS1; AT-V1; AT-V2; FLJ10155; MGC87362;

Entrez Gene ID

UniProt ID

Antigen Description

Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3-5 exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3 overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection.

Pathway

ATM mediated phosphorylation of repair proteins, organism-specific biosystem; ATM mediated response to DNA double-strand break, organism-specific biosystem; Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; DNA Repair, organism-specific biosystem; DNA damage response, organism-specific biosystem;