Anti-NAPSA monoclonal antibody, clone FQS21824(C)

Transient absorption spectra of sodium pyrenesulfonate (NaPSA) after laser excitation at 351 nm were observed in H2O/AOT/isooctane reversed micellar system as well as in aqueous solution. Two-photon ionization of PSA, monitored by detecting the transient absorptions at 455 and 700 nm attributable to the zwitterion and the solvated electron, respectively, was found to hardly occur in the small reversed micelle. The transient absorption at 490 nm has been attributed to the dianion of PSA in which the electron attaches to an unexcited PSA. This peak was observed only in the largest water pool with H2O/AOT = 50. These results are discussed in terms of the location and the number of PSA solubilized in the reversed micelle.

ELF5, an Ets family transcription factor found exclusively in epithelial cells, is expressed in the distal lung epithelium during embryogenesis, then becomes restricted to proximal airways at the end of gestation and postnatally. To test the hypothesis that ELF5 represses distal epithelial differentiation, we generated a transgenic mouse model in which a doxycycline inducible HA-tagged mouse Elf5 transgene was placed under the control of the lung epithelium-specific human SFTPC promoter. We found that expressing high levels of ELF5 during early lung development disrupted branching morphogenesis and produced a dilated epithelium. The effects of ELF5 on morphogenesis were stage-dependent, since inducing the transgene on E16.5 had no effect on branching. ELF5 reduced expression of the distal lung epithelial differentiation markets Erm, Napsa and Sftpc, and type II cell ultrastructural differentiation was immature. ELF5 overexpression did not induce the proximal airway epithelial markers Ccsp and Foxj1, but did induce expression of p63, a marker of basal cells in the trachea and esophagus. High ELF5 levels also induced the expression of genes found in other endodermal epithelia but not normally associated with the lung. These results suggest that precise levels of ELF5 regulate the specification and differentiation of epithelial cells in the lung. (C) 2008 Elsevier Inc. All rights reserved.