Anti-NADK monoclonal antibody Made to order

Name: Anti-NADK monoclonal antibody
SKU: DCABH-12503
Rating: 5 (1 reviews)

Rabbit anti-Human NADK monoclonal antibody for WB, ELISA

COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

A synthetic peptide of human NADK is used for rabbit immunization.

Conjugate

Unconjugated

Target

Alternative Names

NADK; NAD kinase; FLJ13052; poly(P)/ATP NAD kinase; dJ283E3.1; RP1-283E3.6; MGC1900; FLJ37724; FLJ54695; FLJ77769; FLJ78247; FLJ78307; DKFZp686L22239;

Entrez Gene ID

65220

UniProt ID

A0A024R058

Product Background

Antigen Description

NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).

Pathway

Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Nicotinate metabolism, organism-specific biosystem;

Citations

Have you cited DCABH-12503 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Comparison between 'Fuyan' and 'Dongbi' longans in aril breakdown and respiration metabolism

POSTHARVEST BIOLOGY AND TECHNOLOGY

Authors: Lin, Lijuan; Lin, Yixiong; Lin, Hetong; Lin, Mengshi; Ritenour, Mark A.; Chen, Yihui; Wang, Hui; Hung, Yen-Con; Lin, Yifen

Abstract

This study aimed to investigate the difference between 'Fuyan' longan (Dimocarpus longan Lour. cv. Fuyan) fruit and 'Dongbi' longan (Dimocarpus longan Lour. cv. Dongbi) fruit in term of their aril breakdown and respiratory metabolism. As compared to 'Fuyan' longans, the 'Dongbi' longans exhibited lower fruit respiration rate, lower aril breakdown index, higher NADK activity, higher total activities of G-6-PDH and 6-PGDH, higher contents of NADP and NADPH, lower activities of AAO, CCO, PPO, PGI, and SDH, and lower contents of NAD and NADH in aril. These findings suggest that better storability and lower aril breakdown index in 'Dongbi' longans than 'Fuyan' longans might be due to lower activities of respiratory terminal oxidases, and lower respiratory metabolic pathways of EMP-TCA cycle related-enzymes in 'Dongbi' longan aril, which decreased respiratory rate and reduced the consumption of substrates, and subsequently maintained the integrity of the aril of 'Dongbi' longans during storage.

The role of ROS-induced change of respiratory metabolism in pulp breakdown development of longan fruit during storage

FOOD CHEMISTRY

Authors: Lin, Yixiong; Lin, Hetong; Chen, Yihui; Wang, Hui; Lin, Mengshi; Ritenour, Mark A.; Lin, Yifen

Abstract

Compared to the control longans, hydrogen peroxide (H2O2)-treated longans exhibited higher index of pulp breakdown, higher fruit respiration rate, higher activities of pulp phosphohexose isomerase (PGI), succinate dehydrogenase (SDH), cytochrome C oxidase (CCO), ascorbic acid oxidase (AAO) and polyphenol oxidase (PPO), but lower activity of pulp nicotinamide adenine dinucleotide kinase (NADK). H2O2-treated longans also exhibited lower total activities of pulp glucose-6-phosphate dehydrogenase (G-6-PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), lower levels of pulp NADP(H), but higher levels of pulp NAD(H). These data indicated that H2O2-stimulated longan pulp breakdown was owing to a decreased proportion of pentose phosphate pathway (PPP), the increased proportions of Embden-Meyerhof-Parnas pathway (EMP), tricarboxylic acid (TCA) cycle and cytochrome pathway (CCP) in total respiratory pathways. These findings further revealed that H2O2 could enhance respiration rate, and thus accelerate pulp breakdown occurrence and shorten the shelf life of longan fruit.