General Notes
This product (Catalog No. DPABH-20445) is a rabbit-derived polyclonal antibody raised against human N4BP3, targeting the amino acid region 198–544 of the protein. This antibody exhibits cross-reactivity with Human N4BP3 from mouse and rat. It has been affinity purified by Protein A. N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.
DPABH-20445 is suitable for use in Western blot (WB) (1:1,000–1:6,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:50–1:500), IF/ICC (1:50–1:500) applications.
DPABH-20445 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.