Recombinant fusion protein of human MRPS18B (NP_054765.1).
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:500-1:2000 IHC: 1:100-1:200
General Notes
This product (Catalog No. DPABH-11619) is a rabbit-derived IgG polyclonal antibody raised against human MRPS18B. This antibody exhibits cross-reactivity with MRPS18B from mouse and rat. It has been affinity purified by protein A. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. DPABH-11619 is suitable for use in Western blot (WB) (1:500–1:2,000), IHC (1:100–1:200) applications. DPABH-11619 is supplied as a liquid formulation in PBS with 0.02% sodium azide, 50% glycerol, pH7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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