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    3. Anti-Human METTL3 Polyclonal Antibody

    Anti-Human METTL3 Polyclonal Antibody

    Rabbit Anti-Human METTL3 Polyclonal Antibody for WB, IP, IHC

    Bring this labeled antibody directly to your bench!

    pdf COA pdf SDS pdf Datasheet
    Specifications
    Antibody Isotype
    IgG
    Species Reactivity
    Human, Mouse
    Immunogen
    Immunogen range: Between 1 and 50
    Conjugate
    Unconjugated
    Applications
    Application Notes
    Recommended dilution:
    Western Blot: 1:2,000 - 1:10,000
    Immunoprecipitation: 2 - 5 μg/mg lysate
    Immunohistochemistry: 1:200 - 1:1,000.
    Images
    Detection of human METTL3/MT-A70 by immunohistochemistry.
    Sample: FFPE section of human prostate carcinoma.
    Antibody: Antibody used at a dilution of 1:1,000 (0.2µg/ml).
    Detection: DAB
    Detection of human and mouse METTL3/MT-A70 by western blot (h&m) and immunoprecipitation (h).
    Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells.
    Antibodies: Affinity purified antibody used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate.
    Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
    Target
    Alternative Names
    METTL3; methyltransferase like 3; M6A; IME4; Spo8; MT-A70; N6-adenosine-methyltransferase 70 kDa subunit; mRNA m(6)A methyltransferase; methyltransferase-like protein 3; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase;
    Entrez Gene ID
    56339
    UniProt ID
    Q86U44
    Custom Antibody Labeling
    We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More
    Citations
    Have you cited DPABH-04679 in a publication? Let us know and earn a reward for your research.
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    Customer Reviews
    McGraw, S; Vigneault, C; et al. Temporal expression of factors involved in chromatin remodeling and in gene regulation during early bovine in vitro embryo development. REPRODUCTION 133:597-608(2007).
    Wang, Y; Li, Y; et al. N-6-methyladenosine modification destabilizes developmental regulators in embryonic stem cells. NATURE CELL BIOLOGY 16:191-198(2014).
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