Anti-Human MDK (internal) Polyclonal antibody

Objective. Midkine (MK), a heparin-binding growth factor, promotes growth, survival, and migration of various cells. The essential role of MK in migration of inflammatory cells has been shown using mice deficient in the MK gene (Mdk(-/-) mice). We undertook this study to investigate the role of MK in the pathogenesis of rheumatoid arthritis (RA). Methods. MK levels in specimens from patients were determined by enzyme-linked immunosorbent assay, and localization of MK was revealed by immunohistochemical analysis. Susceptibility to antibody-induced arthritis was compared between Mdk(-/-) and wild-type (WT) mice. Osteoclast differentiation was monitored using macrophage-like cells isolated from human synovial tissue and macrophages from mouse bone marrow. Results. MK levels in sera and synovial fluid were increased in most RA patients, indicating a strong correlation between MK expression and RA. MK was expressed in macrophage-like cells and fibroblast-like cells in synovial membranes from the patients. In antibody-induced arthritis, Mdk(-/-) mice seldom developed the disease, while most of the WT mice did. Administration of MK to the Mdk(-/-) mice increased the frequency of antibody-induced arthritis. Migration of inflammatory leukocytes to the synovial membranes in the disease model was suppressed in the Mdk(-/-) mice. Furthermore, MK was found to promote the differentiation of osteoclasts from macrophages. Conclusion. MK participates in each of the two distinct phases of RA development, namely, migration of inflammatory leukocytes and osteoclast differentiation, and is a key molecule in the pathogenesis of RA.

Combining the development need of the embedded training simulator, the embedded development technology based on Matlab and MDK in embedded simulation of water jet propulsion system was studied. Firstly,according to the interaction between the components of the water jet propulsion system, the mathematical model of the system was established in Simulink by means of mechanism modeling, lookup table and C code S function. Then,the RTW toolbox was used to generate the code of the Simulink simulation model, the code integration was completed in the MDK, and the program was download to STM32 as the core of the control system,and to built an embedded experimental platform. Experiments show that the embedded simulation method was effective and feasible, and can be used to construct the on board trainning system(OBTS).