General Notes
This product (Catalog No. DPABH-27255) is a rabbit-derived polyclonal antibody raised against human MAP3K3. This antibody exhibits cross-reactivity with MAP3K3 from mouse. It has been affinity purified by Protein A. MAP3K3 is a human protein-coding gene that encodes mitogen-activated protein kinase kinase kinase 3 (MEKK3), a 626-amino-acid serine/threonine protein kinase (EC 2.7.11.25) essential for signal transduction in cellular stress responses. Located on chromosome 17q23.3, it consists of 19 exons and produces a ubiquitously expressed 4.6-kb transcript, with alternative splicing yielding multiple isoforms including a primary 626-amino-acid isoform; expression is highest in the spleen (RPKM 15.4) and lung (RPKM 14.5). MEKK3 functions as a component of mitogen-activated protein kinase (MAPK) cascades, directly activating the stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) pathway via SEK1 and the extracellular signal-regulated kinase (ERK) pathway via MEK1/2, while not influencing the p38 pathway. It enhances transcription from nuclear factor kappa-B (NF-κB)-dependent reporters and mediates activation of transcription factors including NF-κB, AP-1, and DDIT3, thereby regulating inflammation, immune responses, and cell survival. Additionally, MEKK3 contributes to antiviral immunity, vascular development, and processes like lipopolysaccharide-induced NF-κB activation.
DPABH-27255 is suitable for use in WB 1 (500-1:1000), IF/ICC 1 (100-1:500) applications.
DPABH-27255 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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