Anti-KLHL13 monoclonal antibody, clone 9E2

Antibody Isotype

IgG1

Clone

9E2

Species Reactivity

Human

Immunogen

Recombinant protein corresponding to human KLHL13.

Conjugate

Application Notes

ELISA (1:10000); WB (1:500-1:2000); Immunohistochemistry (1:200-1:1000); Immunofluorescence (1:200-1:1000); Flow Cytometry (1:200-1:400) & The optimal working dilution should be determined by the end user.

Alternative Names

KLHL13; kelch-like 13 (Drosophila); BKLHD2, BTB and kelch domain containing 2, KIAA1309 , KIAA1309; kelch-like protein 13; FLJ10262; BTB and kelch domain containing 2; BKLHD2; MGC74791;

Entrez Gene ID

UniProt ID

Antigen Description

KLHL13 (kelch like 13), also known as BKLHD2 (BTB and kelch domain containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate specific adapter of an E3 ubiquitin protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin mediated proteolysis. Via its BTB and C terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL3 ligase.

Pathway

Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Class I MHC mediated antigen processing & presentation,.