Rabbit Anti-Human IRE1 (a.a. 233-444) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Recombinant human IRE1; ERN1 protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 233-444 aa of BC130405

Conjugate

Application Notes

WB: 1:1000-1:4000

General Notes

This product (Catalog No. DPABH-15710) is a rabbit-derived polyclonal antibody raised against human IRE1, targeting the amino acid region 233–444 of the protein. It has been affinity purified by Protein A. Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595).
DPABH-15710 is suitable for use in Western blot (WB) (1:1,000–1:4,000) application.
DPABH-15710 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

ERN1; IRE1; ERN1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ERN 1

Entrez Gene ID

UniProt ID