Rabbit Anti-IL36RN Polyclonal Antibody (DPABH-00079)
Rabbit Anti-Human IL36RN Polyclonal Antibody for WB
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Recombinant funsion protein containing a sequence corresponding to amino acids 1-155 of human IL36RN (NP_036407.1).
WB: 1:500 - 1:2000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of extracts of various cell lines, using IL36RN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.
IL36RN; interleukin 36 receptor antagonist; FIL1; FIL1D; IL1F5; IL1L1
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Tortola, L; Rosenwald, E; et al. Psoriasiform dermatitis is driven by IL-36-mediated DC-keratinocyte crosstalk. JOURNAL OF CLINICAL INVESTIGATION 122:3965-3976(2012).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.