Application: WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Species Reactivity: Human, Mouse, Rat
Summary
Specifications
Antibody Isotype
IgG
Species Reactivity
Human; Mouse; Rat
Immunogen
Recombinant human IDH3A protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 1-366 aa of BC021967
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:5000-1:50000 IP: 0.5-4.0 μg for 1.0-3.0 mg of total protein lysate IHC: 1:20-1:200 IF/ICC: 1:200-1:800 FC: 0.40 μg per 10^6 cells in a 100 μl suspension
General Notes
This product (Catalog No. DPABH-26084) is a rabbit-derived polyclonal antibody raised against human IDH3A, targeting the amino acid region 1–366 of the protein. This antibody exhibits cross-reactivity with Human IDH3A from mouse and rat. It has been affinity purified by Protein A. IDH3A (isocitrate dehydrogenase 3 (NAD+) alpha) is a protein-coding gene. Among its related super-pathways are Citric acid cycle (TCA cycle) and Metabolic pathways. GO annotations related to this gene include isocitrate dehydrogenase (NAD+) activity and magnesium ion binding. DPABH-26084 is suitable for use in Western blot (WB) (1:5,000–1:50,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:20–1:200), IF/ICC (1:200–1:800), flow cytometry (FC) (0.40 μg per 10^6 cells in a 100 μl suspension) applications. DPABH-26084 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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