Rabbit Anti-Human NFIA (a.a. 41-140) Polyclonal antibody

Name: Rabbit Anti-Human NFIA (a.a. 41-140) Polyclonal antibody
SKU: CPBT-55956RH

Rabbit Anti-Human NFIA (a.a. 41-140) Polyclonal antibody for IHC-P, IHC-F, IF

COA

SDS

Datasheet

Key features and details

Target: NFIA
Host: Rabbit
Application: IHC-P, IHC-F, IF
Species Reactivity: Rat, Human, Mouse, Chicken, Dog, Pig, Cow, Sheep

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Rat; Human; Mouse; Chicken; Dog; Pig; Cow; Sheep

Immunogen

KLH conjugated synthetic peptide derived from human NFIA/NF-1: 41-140/509

Conjugate

Unconjugated

Applications

Application Notes

IHC-P: 1:100-500
IHC-F: 1:100-500
IF: 1:100-500

General Notes

This product (Catalog No. CPBT-55956RH) is a rabbit-derived IgG polyclonal antibody raised against human NFIA, targeting the amino acid region 41–140 of the protein. This antibody exhibits cross-reactivity with NFIA from rat, mouse, chicken, dog, pig, cow, and sheep. It has been affinity purified by protein A. Nuclear factor 1 A-type (NFIA) is a crucial DNA-binding transcription factor and member of the NFI family, essential for regulating gene expression during development, particularly in the brain, lung, and, spinal cord. It functions as a dimer to activate or repress transcription and controls the neural stem cell switch from neurogenesis to gliogenesis.
CPBT-55956RH is suitable for use in IHC-P (1:100–1:500), IHC-F (1:100–1:500), IF (immunofluorescence) (1:100–1:500) applications.
CPBT-55956RH is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

NFIA; KIAA1439; Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein; NFIB; Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/B; NF-I/B; NFI-B; TGGCA-binding protein; NFIC; NFI; Nuclear factor 1 C-type; NF1-C; Nuclear factor 1/C; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/C; NF-I/C; NFI-C; TGGCA-binding protein; NFIX; Nuclear factor 1 X-type; NF1-X; Nuclear factor 1/X; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/X; NF-I/X; NFI-X; TGGCA-binding protein

Entrez Gene ID

4774

UniProt ID

Q12857

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

NOVEL ONCOGENIC ROLE FOR NFIA IN GLIOMA

NEURO-ONCOLOGY

Authors: Hoxha, Edlira; Andreas, Kloetgen; Song, Hae-Ri

Abstract

Neuroplastin- mediates S100A8/A9-induced lung cancer disseminative progression

MOLECULAR CARCINOGENESIS

Authors: Sumardika, I. Wayan; Chen, Youyi; Tomonobu, Nahoko; Kinoshita, Rie; Ruma, I. Made Winarsa; Sato, Hiroki; Kondo, Eisaku; Inoue, Yusuke; Yamauchi, Akira; Murata, Hitoshi; Yamamoto, Ken-ichi; Tomida, Shuta; Shien, Kazuhiko; Yamamoto, Hiromasa; Soh, Junichi; Futami, Junichiro; Putranto, Endy Widya; Hibino, Toshihiko; Nishibori, Masahiro; Toyooka, Shinichi; Sakaguchi, Masakiyo

Abstract

Compiling evidence indicates an unusual role of extracellular S100A8/A9 in cancer metastasis. S100A8/A9 secreted from either cancer cells or normal cells including epithelial and inflammatory cells stimulates cancer cells through S100A8/A9 sensor receptors in an autocrine or paracrine manner, leading to cancer cell metastatic progression. We previously reported a novel S100A8/A9 receptor, neuroplastin- (NPTN), which plays a critical role in atopic dermatitis when it is highly activated in keratinocytes by an excess amount of extracellular S100A8/A9 in the inflammatory skin lesion. Interestingly, our expression profiling of NPTN showed significantly high expression levels in lung cancer cell lines in a consistent manner. We hence aimed to determine the significance of NPTN as an S100A8/A9 receptor in lung cancer. Our results showed that NPTN has strong ability to induce cancer-related cellular events, including anchorage-independent growth, motility and invasiveness, in lung cancer cells in response to extracellular S100A8/A9, eventually leading to the expression of a cancer disseminative phenotype in lung tissue in vivo. Mechanistic investigation revealed that binding of S100A8/A9 to NPTN mediates activation of NFIA and NFIB and following SPDEF transcription factors through orchestrated upstream signals from TRAF2 and RAS, which is linked to anchorage-independent growth, motility and invasiveness. Overall, our results indicate the importance of the S100A8/A9-NPTN axis in lung cancer disseminative progression and reveal a pivotal role of its newly identified downstream signaling, TRAF2/RAS-NFIA/NFIB-SPDEF, in linking to the aggressive development of lung cancers.