Anti-FGG polyclonal antibody

Species Reactivity

Human

Immunogen

Asynthetic peptide containing the sequence unique to the γ′ chain (VRPEHPAETEYDSLYPEDDL) conjugated to keyhole limpet hemocyanin carrier.

Conjugate

Alternative Names

Fg-γ′; FGG;fibrinogen gamma chain; fibrinogen, gamma polypeptide; PRO2061; OTTHUMP00000197057;OTTHUMP00000197058; OTTHUMP00000197059; OTTHUMP00000197060; Fibrinogen GammaPrime

Entrez Gene ID

UniProt ID

Antigen Description

Human fibrinogen is a 340 kDa plasma protein produced in theliver. Plasma concentrations are typically 1.7 – 3.5 g/L (5-10 μM). The intactfibrinogen molecule consists of two identical subunits, each consisting ofthree non-identical polypeptide chains denoted as Aα, Bβ and γ. The letters Aand B in the Aα and Bβ chains designate, respectively, fibrinopeptide A (FpA,residues 1-16), and fibrinopeptide B (FpB, residues 1-14), which are cleavedby thrombin upon conversion of fibrinogen to fibrin. The fibrin monomerspolymerize in a half-overlap fashion to form insoluble fibrin fibrils. Thepolymerised fibrin is subsequently stabilized by activated Factor XIII thatforms amide linkages between γ chains and, to a lesser extent, α chains of thefibrin molecules. Proteolysis of fibrinogen by plasmin initially liberatesC-terminal residues from the Aα chain to produce fragment X (intact D-E-D, whichis still clottable). Fragment X is further degraded to nonclottable fragmentsY (D-E) and D. Fragment Y can be digested into its constituent D and Efragments. Proteolysis of crosslinked fibrin by plasmin results in fragmentDD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked viathe γ chains), fragment E (central E domain) as well as DDE in which fragmentE is noncovalently associated with DD. The molecular weights of the
cleavage fragments produced from human crosslinked fibrin are: 184kDa for fragment DD, 92 kDa for D, 50 kDa for E, 1.54 kDa for FpA and 1.57kDa for FpB. Most of the fibrinogen in the circulation consists of 2 copiesof each chain (Aα2, Bβ2, γA2), but in normalplasma approximately 10% of the fibrinogen molecules contain one γA chainand one variant γ chain (termed γ′), in which the c-terminal AGDV residuesare replaced with the amino acid sequence VRPEHPAETEYDSLYPEDDL. This variant
fibrinogen is commonly referred to as fibrinogen gamma prime (γA/γ′)but has also been called fibrinogen 2 or peak 2 fibrinogen because it elutesseparately from fibrinogen 1 (γA2) by ion exchange chromatography.Residues 414-427 of the γ′ chain of fibrin gamma prime (contain ahigh-affinity binding site for exosite II of thrombin, which allows theactive site of bound thrombin to remain available to interact with substrateswhile demonstrating resistance to heparin mediated inhibition by antithrombin.

Pathway

Blood ClottingCascade; Common Pathway; Complement and coagulation cascades; Formation ofFibrin Clot (Clotting Cascade); GRB2:SOS provides linkage to MAPK signalingfor Intergrins;
Glucocorticoidreceptor regulatory network; Hemostasis; IL6-mediated signaling events; IntegrinalphaIIb beta3 signaling; Integrin cell surface interactions; PlateletAggregation (Plug Formation); Platelet activation, signaling and aggregation;Platelet degranulation; Response to elevated platelet cytosolic Ca2+; SignalT