Rabbit Anti-HPSE2 Polyclonal Antibody (DPABH-24997)
Rabbit Anti-Human HPSE2 Polyclonal Antibody for WB, IHC
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Human, Mouse, Rat
Recombinant funsion protein containing a sequence corresponding to amino acids 42-242 of human HPSE2 (NP_068600.4).
WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Immunohistochemistry of paraffin-embedded human prostate using HPSE2 antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HPSE2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
HPSE2; heparanase 2 (inactive); UFS; HPA2; HPR2; UFS1
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Rodrigues, LMR; de Oliveira, LZ; et al. Expression of Heparanase Isoforms in Intervertebral Discs Classified According to Pfirrmann Grading System for Disc Degeneration. SPINE 38:1112-1118(2013).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.