Clinical relevance of major histocompatibility complex class I chain-related molecule A (MICA) antibodies in live donor renal transplantation - Indian Experience
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
Authors: Baranwal, Ajay Kumar; Bhat, Deepali K.; Goswami, Sanjeev; Agarwal, Sanjay Kumar; Kaur, Gurvinder; Mehra, Narinder
Abstract
Antibody-mediated rejections (AMR) in the absence of circulating anti-HLA-DSA have highlighted the role of non-HLA antibodies, particularly those directed against endothelial cells. Of these, MICA (major histocompatibility complex class I chain-related molecule A) antibodies are the most notable and important because of their potential in promoting graft rejections. Limited studies have focused on the impact of MICA donor-specific antibodies (DSA) on graft outcome as compared to those that are not donor-specific (NDSA). We evaluated pre- and post-transplant sera at POD 7, 30, 90, 180 and the time of biopsy from 206 consecutive primary live donor renal transplant recipients for anti-MICA and anti-HLA antibodies using single antigen bead assay on a Luminex platform. Recipients who developed MICA antibodies and their donors were phenotyped for MICA alleles. For the purpose of antibody analysis, patients were categorized into three major groups: biopsy-proven AMR, acute cellular rejection (ACR) and those with no rejection episodes (NRE). During the mean follow-up period of 17.37 +/- 6.88 months, 16 of the 206 recipients developed AMR, while ACR was observed in only 13 cases. A quarter (25%) of the AMR cases had anti-MICA antibodies as compared to 7.7% of those experiencing ACR and 6.2% of the NRE group. Allelic typing revealed that all MICA Ab +ve AMR cases were due to the presence of donor-specific antibodies. MICA-DSA even in the absence of HLA-DSA was significantly associated with AMR but not with ACR when compared with the NRE group (P = <.01).
Genetic diversity of HLA system in seven populations from Veracruz, Mexico: Veracruz city, Coatzacoalcos, Cordoba, Orizaba, Poza Rica, Xalapa and rural Veracruz
HUMAN IMMUNOLOGY
Authors: Barquera, Rodrigo; Lopez-Gil, Concepcion; Acuna-Alonzo, Victor; del Rosario Vega-Martinez, Maria; Jesus Rodriguez-Munguia, Tirzo; Cesar Martinez-Alvarez, Julio; Arrieta-Bolanos, Esteban; Clayton, Stephen; del Rocio Ramos-de la Cruz, Flor; Iraiz Hernandez-Zaragoza, Diana; Bravo-Acevedo, Alicia; Benitez-Arvizu, Gamaliel; Araceli Arrazola-Garcia, Maria; Aquino-Rubio, Guadalupe; Juarez-Barreto, Vicencio; Mendez-Mani, Patricia; Veronica Vazquez-Castillo, Tannya; Salgado-Galicia, Norma; Solis-Martinez, Raul; de los Angeles Pavon-Vargas, Maria; Zuniga, Joaquin; Yunis, Edmond J.; Bekker-Mendez, Carolina; Granados, Julio
Abstract
We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 1113 Mexicans from the state of Veracruz living in the cities of Coatzacoalcos (N = 55), Orizaba (N = 60), Cordoba (N = 56), Poza Rica (N = 45), Veracruz (N = 171), Xalapa (N = 187) and rural communities (N = 539) to obtain information regarding allelic and haplotypic frequencies. We found that the most frequent haplotypes include 12 Native American haplotypes. Admixture estimates revealed that the main genetic components are Native American (64.93 +/- 1.27% by ML; 55.10% of Native American haplotypes) and European 26.56 +/- 0.89% by ML; 28.38% of European haplotypes), and a relatively high African genetic component (8.52 +/- 1.82% by ML; 8.78% of African haplotypes).
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