Anti-HIV type 1 Integrase Monoclonal antibody, Clone JO-3

Antibody Isotype

IgG1

Clone

JO-3

Species Reactivity

HIV

Immunogen

Bacterially expressed, Hexahistidine amino-terminal tagged HIV-1 integrase protein (clade B, HXB-3 isolate)

Conjugate

Application Notes

ELISA: 0.8 μg/ml-2 μg/ml;
Western blot: 0.4 μg/ml-1.5 μg/ml;
Immunofluorescence: 1.5 μg/ml-3 μg/ml;
Immunoprecipitation assays: 5 μg/ml-10 μg/ml.

Alternative Names

Human immunodeficiency virus; HIV; Human immunodeficiency virus 1; Group VI; Retroviridae; Lentivirus; Integrase; IN; p31; HIV-1 integrase; HIV integrase; Integrase HIV-I; HIV-1 IN; Retroviral integrase

Antigen Description

Integrase is an enzyme, a product of the HIV1 Gag/Pol gene; the other two enzymes being reverse transcriptase and protease. The human immunodeficiency virus (HIV-1) uses an enzyme, a so-called integrase, to carry out the integration of its viral DNA into the host chromosome thereby tricking the host cell machinery into making viral proteins. The HIV-1 integrase is a protein of 32 kDa and is composed of three domains. No cellular homologue of HIV integrase has been described, so potential inhibitors to this enzyme could be relatively nontoxic. Integration of viral DNA into a chromosome of the host cell is an essential step in the retroviral life cycle. The process is catalyzed by the viral enzyme integrase (IN) through 3 steps : first step, two nucleotides are removed from the 39 ends of the viral DNA (39-end processing); second step, the recessed 39 ends of the viral DNA are then joined to 59 staggered sites in the target DNA in a concerted cleavage and ligation reaction (DNA joining); last step, integration is completed by repair of the short gaps flanking the viral DNA intermediate and subsequent joining of the 59 ends of viral DNA to the target DNA.