Anti-HIST2H2BF monoclonal antibody, clone nBcdbn 75276

Antibody Isotype

IgG2b

Clone

nBcdbn 75276

Species Reactivity

Mouse; Human

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H2B.

Conjugate

Application Notes

ICC/IF: 1 μg/ml; WB: 1 - 5 μg/ml; Flow Cyt: 1μg/10⁶ cells; IP: 5 μg/ml; ChIP: 5 μg for μg of chromatin.

Alternative Names

HIST2H2BF; histone cluster 2, H2bf; histone 2, H2bf; histone H2B type 2-F; FLJ35099; FLJ56780; FLJ56787; H2B2F_HUMAN; MGC131639

Entrez Gene ID

UniProt ID

Antigen Description

In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved.

Pathway

Systemic lupus erythematosus, organism-specific biosystem; Systemic lupus erythematosus, conserved biosystem;