Anti-HIF3A monoclonal antibody, clone 5D3

Antibody Isotype

IgG1

Clone

5D3

Species Reactivity

Human

Immunogen

Recombinant fragment corresponding to Human HIF3 alpha aa 355-669. Produced in E.coli.Sequence: QTEQHSRRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAA DPRRFCSPDLRRLLGPILDGASVAATPSTPLATRHPQSPLSADLPDELPV GTENVHRLFTSGKDTEAVETDLDIAQDADALDLEMLAPYISMDDDFQLNA SEQLPR

Conjugate

Application Notes

WB: 1/200; IHC-P: 1/150;

Alternative Names

HIF3A; hypoxia inducible factor 3, alpha subunit; hypoxia-inducible factor 3-alpha; bHLHe17; IPAS; MOP7; PASD7; HIF3-alpha-1; member of PAS protein 7; inhibitory PAS domain protein; PAS domain-containing protein 7; basic-helix-loop-helix-PAS protein MOP7;

Entrez Gene ID

UniProt ID

Antigen Description

Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5-TACGTG-3 within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.

Pathway

Hypoxic and oxygen homeostasis regulation of HIF-1-alpha, organism-specific biosystem;