General Notes
This product (Catalog No. DPABH-21279) is a rabbit-derived polyclonal antibody raised against human GTF2F2, targeting the amino acid region 1–249 of the protein. It has been affinity purified by Protein A. GTF2F2 is a protein-coding gene in humans that encodes the 30 kDa subunit, known as RAP30 or TFIIFβ, of the general transcription factor IIF (TFIIF), a heterodimeric complex crucial for the assembly of the preinitiation complex and the regulation of RNA polymerase II (Pol II)-dependent transcription initiation and elongation. TFIIF, composed of GTF2F2 and its partner subunit GTF2F1 (RAP74), binds directly to Pol II and facilitates its recruitment to promoter DNA in collaboration with other general transcription factors like TFIIB, enabling basal and activated transcription in eukaryotic cells.
DPABH-21279 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-21279 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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