General Notes
This product (Catalog No. DPABH-04031) is a rabbit-derived polyclonal antibody raised against human GLUD1, targeting the amino acid region 334–556 of the protein. This antibody exhibits cross-reactivity with Human GLUD1 from mouse and rat. It has been affinity purified by Protein A. Glutamate dehydrogenase (GDH), also known as GLUD, is an enzyme ubiquitous across all living organisms and localized to the mitochondria in eukaryotes that catalyzes the reversible oxidative deamination of L-glutamate to α-ketoglutarate (α-KG) and ammonia, utilizing NAD⁺ or NADP⁺ as cofactors. This reaction serves as a critical junction between amino acid catabolism and the tricarboxylic acid (TCA) cycle, enabling the integration of nitrogen and carbon metabolism. In vivo, GDH predominantly operates in the catabolic direction, producing α-KG for energy production and releasing ammonia for further processing, such as in the urea cycle. Structurally, GDH typically assembles as a homohexamer in animals, composed of six subunits each approximately 500 amino acids in length, featuring an NAD-binding domain and, in higher organisms, a unique regulatory "antenna" region that influences allosteric control. GDHs are classified into small (∼50 kDa subunits) and large (∼115–180 kDa) subfamilies, with the latter prevalent in prokaryotes and lower eukaryotes and characterized by additional N- and C-terminal extensions that enhance oligomerization and regulation. In humans, two isoforms exist: the housekeeping GLUD1, expressed widely, and the hominoid-specific GLUD2, primarily in neural and testicular tissues, arising from a retroposition event on the X chromosome.
DPABH-04031 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-04031 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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