Anti-GFP tag antibody, clone HU8423

Antibody Isotype

IgG2a

Clone

HU8423

Immunogen

Recombinant fragment within Aequorea victoria GFP aa 1-238. The exact sequence is proprietary.

Conjugate

Application Notes

IP: Use at 5 μg/mg of lysate. ELISA: Use a concentration of 0.1 - 0.2 μg/ml; WB: 1/5000 - 1/20000.

Alternative Names

GFP; green fluorescent protein;

Entrez Gene ID

UniProt ID

Antigen Description

Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Biophysicochemical properties: Absorption: Abs(max)=395 nm Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.