General Notes
This product (Catalog No. DPABH-00099) is a rabbit-derived polyclonal antibody raised against human GAP43, targeting the amino acid region 1–238 of the protein. This antibody exhibits cross-reactivity with Human GAP43 from mouse and rat. It has been affinity purified by Protein A. The neuronal growth-associated protein GAP43 is known as neuromodulin, B-50, P-57, F1, and pp46. Deficiency of GAP43 in mice results in death early in the postnatal period. GAP43 is one of the main substrates for protein kinase C in the brain. GAP43 is an intracellular growth-associated protein that appears to assist neuronal pathfinding and branching during development and regeneration and may contribute to presynaptic membrane changes in the adult, leading to neurotransmitter release, endocytosis, and synaptic vesicle recycling, long-term potentiation, spatial memory formation, and learning. The predicted molecular weight of about 25 kDa is much lower than the apparent observed molecular weight of 43 kDa on SDS-PAGE gels, and this occurs because the highly charged nature of GAP43 causes it to bind less than the average amount of SDS per amino acid, and because the protein has an elongated structure.
DPABH-00099 is suitable for use in Western blot (WB) (1:2,000–1:10,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:50–1:500), IF-P (1:50–1:500), IF/ICC (1:200–1:800) applications.
DPABH-00099 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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