Anti-GALM (full length) polyclonal antibody

Objective. Many physical activity interventions do not reach those people who would benefit the most from them. The Groningen Active Living Model (GALM) was successful in recruiting sedentary and underactive older adults. Method. In the fall of 2000 older adults in three municipalities in the Netherlands received written information, were visited at home and, if eligible according to the GALM recruitment criteria, filled in the Stages of Change questionnaire and the Voorrips physical activity questionnaire. Results. By using the strategy we succeeded in including 12.3% of the older adults (315 of the 2551 qualifying participants), 79.4% of whom could be indeed regarded as sedentary or underactive. These results can be considered in line with results described in the literature. The cost of successfully recruiting an older adult was estimated at $84. Conclusions. The GALM recruitment strategy is a potentially useful and effective method for reaching Community-dwelling sedentary and underactive older adults. (C) 2008 Elsevier Inc. All rights reserved.

The aim of this study was to investigate the acid tolerance response (ATR) in Lactobacillus casei by a combined physiological and proteomic analysis. To optimize the ATR induction, cells were acid adapted for 1 h at different pHs, and then acid challenged at pH 3.5. The result showed that acid adaptation improved acid tolerance, and the highest survival was observed in cells adapted at pH 4.5 for 1 h. Analysis of the physiological data showed that the acid-adapted cells exhibited higher intracellular pH (pH(i)), intracellular NH4 (+) content, and lower inner permeability compared with the cells without adaptation. Proteomic analysis was performed upon acid adaptation to different pHs (pH 6.5 vs. pH 4.5) using two-dimensional electrophoresis. A total of 24 proteins that exhibited at least 1.5-fold differential expression were identified. Four proteins (Pgk, LacD, Hpr, and Galm) involved in carbohydrate catabolism and five classic stress response proteins (GroEL, GrpE, Dnak, Hspl, and LCAZH_2811) were up-regulated after acid adaptation at pH 4.5 for 1 h. Validation of the proteomic data was performed by quantitative RT-PCR, and transcriptional regulation of all selected genes showed a positive correlation with the proteomic patterns of the identified proteins. Results presented in this study may be useful for further elucidating the acid tolerance mechanisms and may help in formulating new strategies to improve the industrial performance of this species during acid stress.