Rabbit Anti-Human AFAF (a.a. 20-121) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Recombinant human C9orf11 protein; Source: E. coli derived, PET28a; Tag: 6*His; Domain: 20-121 aa of BC137351

Conjugate

Application Notes

WB: 1:500-1:2000
IF/ICC: 1:200-1:800

General Notes

This product (Catalog No. DPABH-18041) is a rabbit-derived polyclonal antibody raised against human AFAF, targeting the amino acid region 20–121 of the protein. It has been affinity purified by Protein A. The AFAF gene (also known as EQTN and C9orf11) encodes a membrane protein that is specifically localized to the acrosomal membrane. AFAF is involved in the process of fertilization and acrosome biogenesis. Expression analysis demonstrates that AFAF is highly expressed in the testis, although minor expression was seen in other tissues. AFAF has 3 isoforms with molecular weights of 14, 29, and 33 kDa. However, one article detected molecular weight between 40-65 kDa in mouse testis (PMID: 19605790).
DPABH-18041 is suitable for use in Western blot (WB) (1:500–1:2,000), IF/ICC (1:200–1:800) applications.
DPABH-18041 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

AFAF; C9orf11; EQTN; Equatorin

Entrez Gene ID

UniProt ID