Rabbit Anti-Human EPHA7 (internal) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

A synthesized peptide derived from human EPHA7, corresponding to a region within the internal amino acids.

Conjugate

Application Notes

WB 1:500-1:2000
IF/ICC 1:100-1:500

General Notes

This product (Catalog No. DPABH-25463) is a rabbit-derived polyclonal antibody raised against human EPHA7. This antibody exhibits cross-reactivity with EPHA7 from mouse and rat. It has been affinity purified by Protein A. Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.
DPABH-25463 is suitable for use in WB 1 (500-1:2000), IF/ICC 1 (100-1:500) applications.
DPABH-25463 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Entrez Gene ID

UniProt ID