Up-regulated microRNA-299 corrected with poor prognosis of glioblastoma multiforme patients by targeting ELL2
JAPANESE JOURNAL OF CLINICAL ONCOLOGY
Authors: Huang, Qian; Zhang, Xin-Wen; Ma, Yu-Shui; Lu, Gai-Xia; Xie, Ru-Ting; Yang, Hui-Qiong; Lv, Zhong-Wei; Zhong, Xiao-Ming; Liu, Tao; Huang, Shi-Xiong; Fu, Da; Xie, Chun
Abstract
An increasing understanding of the genes and molecular pathways of glioblastoma multiforme (GBM) can provide us a useful insight for the development of more effective targeted therapeutic. To investigate the expression and clinical significance of miR-299 and its target genes in GBM, the expression levels of miR-299 and its target gene in human normal brain tissues and GBM were analyzed in silico using genes microarray and hierarchical clustering analysis followed by validation with quantitative RT-PCR. Our results show that miR-299 is up-regulated in GBM patients. Moreover, patients with low miR-299 expression had longer overall survival (OS) compared with those with high miR-299 expression. RNA polymerase II elongation factor, ELL2, was identified as a miR-299 direct target. High expression of ELL2 together with miR-299 down-regulation correlated with a shorter median OS. Our results provide the first evidence that ELL2 is a direct target of miR-299 and increased ELL2 expression and down-regulation of miR-299 are associated with GBM progression and poor prognosis in patients, suggesting that ELL2 and miR-299 might have potential prognostic value and be used as tumor biomarkers for the diagnosis of patients with GBM.
Data supporting the functional role of Eleven-nineteen Lysine-rich Leukemia 3 (ELL3) in B cell lymphoma cell line cells
DATA IN BRIEF
Authors: Alexander, Lou -Ella M. M.; Watters, January; Reusch, Jessica A.; Maurin, Michelle; Nepon-Sixt, Brook S.; Vrzalikova, Katerina; Alexandrow, Mark G.; Murray, Paul G.; Wright, Kenneth L.
Abstract
The data presented here are related to the research article entitled "Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival" (Alexander et al., 2017) [1]. The cited research article characterizes Eleven nineteen Lysine-rich Leukemia 3 (ELL3) expression in the B cell compartment and functional dependence in B lymphoma cell lines. This data report describes the mRNA expression pattern in a panel of cell lines representing the B cell compartment, supplementing the protein expression data presented in the associated research report. In addition, a reanalysis is presented of publicly available mRNA expression data from primary murine B cells to reveal dynamic regulation of the ELL family members post LPS stimulation (Barwick et al., 2016) [2]. The effect of ELL3 depletion on cell morphology, latent Epstein Barr Virus (EBV) lytic replication and differentiation markers in a Burkitt's lymphoma (BL) cell line cells are presented. (C) 2017 The Authors. Published by Elsevier Inc.
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