Anti-eIF4E monoclonal antibody, clone 6E22

Antibody Isotype

IgG1

Clone

6E22

Species Reactivity

Mouse; Human

Immunogen

Recombinant full length protein corresponding to Human eIF4E. Produced in HEK293T cells.Database link: P06730

Conjugate

Application Notes

ICC/IF: 1/100 - 1/500; WB: 1/1000; IP: 6 μg/mg.

Alternative Names

EIF4E; eukaryotic translation initiation factor 4E; EIF4EL1, EIF4F; EIF4E1; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1; CBP; EIF4F; EIF4EL1; MGC111573;

Entrez Gene ID

UniProt ID

Antigen Description

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Pathway

Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Deadenylation of mRNA, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem;