Anti-EDA monoclonal antibody, clone 285907

Hypohidrotic ectodermal dysplasias (HED) constitute a group of genetic disorders that affect ectodermal derivatives such as sweat glands, sebaceous glands, hair, and teeth. The vast majority of cases of HED are caused by a recessive mutation of the EDA gene located in the X chromosome. In these cases, affected individuals are usually male and have alopecia and hypotrichosis with characteristic distribution, in addition to malformed teeth and fewer than normal. From a canine HED isolated case (proband) andc in order to verify if this emerged from a new mutation, it was possible to construct a pedigree with 5 generations and 93 individuals representing an extended and informative family. The proband's mother crossed with 2 different males and generated 33 descendants in 9 gestations: 1 affected male (proband), 15 normal males, and 17 normal females, which together can be considered as 1 sibship. Through Bayesian inference, it was possible to establish that this case originated from a new mutation, with a 99.99% probability of the mother of the proband not being a carrier. (C) 2020 Elsevier Inc. All rights reserved.

Microencapsulation of healing agents has been applied to realize the self-healing function of polymeric composites. In practice, however, microencapsulation of a liquid amine is very complicated because it is soluble in water and most organic solvents. In this paper, we report a new approach to preparing amine microcapsules that constitute a dual-microcapsule epoxy-amine self-healing system for epoxy composites. Ethylenediamine (EDA)-containing microcapsule with epoxy-EDA shell was successfully synthesized via interfacial polymerization in a water-in-oil emulsion. There were three key factors to solve the problem of microencapsulated a liquid amine, including preparation of epoxy-EDA shell prepolymer, a small amount of deionized water into core material, and polydimethylsiloxane as the continuous phase. A series of methods including Fourier transform infrared, differential scanning calorimetry, optical microscope, and scanning electron microscope were carried out to prove that microcapsules were suitable for preparing self-healing composites. Healing efficiency of self-healing epoxy achieved to 80.4% with 12 wt% epoxy-containing microcapsules and 8 wt% EDA-containing microcapsules. In general, this work provides a novel preparation method of liquid amine microcapsules. This novel and effective self-healing system may be useful for the development of epoxy-based structural composites for various applications. (C) 2020 The Authors. Published by Elsevier Ltd.