Anti-DHFR monoclonal antibody (CAB-3135MH)

Mouse anti-DHFR monoclonal antibody for ELISA, WB, IF Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2a
Clone
MAAG582
Species Reactivity
Human
Immunogen
DHFR (AAH03584, 88 a.a. ~ 188 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Unconjugated

Applications


Application Notes
WB: 1:500-1000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
DHFR; dihydrofolate reductase; DYR; DHFRP1
Entrez Gene ID
UniProt ID

Product Background


Pathway
Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; E2F mediated regulation of DNA replication, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem;

Citations


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References


Cacciatore, JJ; Chasin, LA; et al. Gene amplification and vector engineering to achieve rapid and high-level therapeutic protein production using the Dhfr-based CHO cell selection system. BIOTECHNOLOGY ADVANCES 28:673-681(2010).
Ryu, CJ; Park, HR; et al. Characterization of expression of a chimeric antibody using a dihydrofolate reductase (DHFR) procedure. ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 8 :329-337(1997).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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