Anti-DHFR monoclonal antibody, clone MAAG582

Antibody Isotype

IgG2a

Clone

MAAG582

Species Reactivity

Human

Immunogen

DHFR (AAH03584, 88 a.a. ~ 188 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

Application Notes

WB: 1:500-1000

Alternative Names

DHFR; dihydrofolate reductase; DYR; DHFRP1;

Entrez Gene ID

UniProt ID

Antigen Description

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Pathway

Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; E2F mediated regulation of DNA replication, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem;