Elevated serum DAND5 is associated with metastasis and predicts poor prognosis in colorectal cancer
UNITED EUROPEAN GASTROENTEROLOGY JOURNAL
Authors: Miao, Xiaofei; Zhang, Ye; Sun, Jialin; Cui, Songkui; Meng, Qingyang; Zhu, Kuiyu; Hu, Xingqian; Wang, Tong
Abstract
Background: The biologic and clinical significance of DAND5 remains unknown in colorectal cancer (CRC). Objective: Herein, we investigated the function of DAND5 and evaluated its clinical significance in both serum and matched primary tumors in patients with CRC. Methods: The role of DAND5 was explored in CRC cells and clinical significance of DAND5 was investigated in CRC patients (n=217) and healthy controls (n=63). Results: Knockdown of DAND5 significantly decreased CRC cell proliferation, migration and invasion partly associated with epithelial-mesenchymal transition phenotype. Serum DAND5 levels in CRC were significantly higher than in normal controls and accurately distinguished CRC from healthy subjects. High serum DAND5 levels were significantly correlated with tumor differentiation, large tumor size, advanced Tumor Node Metastasis (TNM) stage, lymph node and liver metastasis, high carcinoembryonic antigen level, recurrence, poor overall and disease-free survival. Serum DAND5 level, together with lymph node metastasis, were independent prognostic factors for CRC patients. High DAND5 protein expression in CRC tissues was increased according to TNM stage. A significant positive correlation existed between serum DAND5 levels and matched DAND5 expression in CRC tissues. Conclusion: Our data provide novel evidence for the clinical significance of DAND5 as a potential biomarker for CRC prognosis.
Identification and expression analysis of BMP signaling inhibitors genes of the DAN family in amphioxus
GENE EXPRESSION PATTERNS
Authors: Le Petillon, Yann; Oulion, Silvan; Escande, Marie-Line; Escriva, Hector; Bertrand, Stephanie
Abstract
Bone morphogenetic proteins (BMPs) are members of the Transforming Growth Factor-beta (TGF-beta family implicated in many developmental processes in metazoans such as embryo axes specification. Their wide variety of actions is in part controlled by inhibitors that impede the interaction of BMPs with their specific receptors. Here, we focused our attention on the Differential screening-selected gene Aberrative in Neuroblastoma (DAN) family of inhibitors. Although they are well-characterized in vertebrates, few data are available for this family in other metazoan species. In order to understand the evolution of potential developmental roles of these inhibitors in chordates, we identified the members of this family in the cephalochordate amphioxus, and characterized their expression patterns during embryonic development. Our data suggest that the function of Cerberus/Dand5 subfamily genes is conserved among chordates, whereas Gremlin1/2 and NBL1 subfamily genes seem to have acquired divergent expression patterns in each chordate lineage. On the other hand, the expression of Gremlin in the amphioxus neural plate border during early neurulation strengthens the hypothesis of a conserved neural plate border gene network in chordates. (C) 2013 Elsevier B.V. All rights reserved.
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