Anti-LAMP1 monoclonal antibody

The lysosome-associated membrane proteins (LAMPs), particularly LAMP1 and LAMP2, are crucial components of lysosomal membranes, constituting approximately 50% of the total lysosomal membrane proteins. These proteins feature a LAMP domain of around 200 amino acids, along with multiple N-glycosylation and O-glycosylation sites. LAMP1, also referred to as CD107a or LAMP-A, is a type I transmembrane protein predominantly found in lysosomal and endosomal membranes, with lesser amounts in cytoplasmic membranes. LAMP1 plays a crucial role in preserving the functionality of lysosomes and acts as a key marker for these organelles. Additionally, it has a significant impact on tumor development by modulating various processes, including autophagy, metastasis, and invasion in cancer cells. Due to its involvement in these critical functions, LAMP1 can serve as an important indicator for assessing tumor progression and prognosis.

Figure 1. Lysosomal proteins in cell death mechanisms
(Source: Mrschtik M, et al. 2015)

The human LAMP1 gene is located in the chromosome 13q34 region and is highly conserved in evolution. The LAMP1 protein contains 416 amino acids and the relative molecular mass of the polypeptide backbone is 40-45kDa, which is increased to 120kDa by glycosylation. The LAMP1 protein is divided into three parts by the lysosomal membrane, with the C-terminus on the cytoplasmic side and the N-terminus within the lysosome. LAMP1 on the luminal surface of the lysosome is divided into two homologous regions by a proline- and serine-rich hinge-like structure. In addition, there are 18 N-glycosylation sites and 6 O-glycosylation sites on the LAMP1 protein that add sugar chains to maintain the stability of the LAMP1 protein on the lysosomal membrane.

LAMP1 not only has a role in maintaining the structural integrity of lysosomes and pH stability within lysosomes, but also participates in a variety of intracellular physiological processes, such as the regulation of lysosomal cytosol and cholesterol transport. LAMP1 has also been associated with the development of diseases such as Alzheimer's disease and lysosomal storage disease. In tumor cells, the expression of LAMP1 can enhance the migration ability of cells during metastasis, thus affecting the metastasis and invasion potential of tumor cells. In different types of tumors, the change of LAMP1 expression level can indicate whether the tumor prognosis is good.

Anti-lysosome-associated membrane protein 1 monoclonal antibody