Anti-Chicken IgY Fab monoclonal antibody, clone M90A90

Egg yolk immunoglobulin (IgY) is a superior functional equivalent to mammalian IgG. However, the preparation of refined and highly purified IgY is still attributed as difficult task. Protein M (a transmembrane protein from human mycoplasma) has been newly demonstrated as an ideal affinity regent for mammalian antibody purification. This study aimed to evaluate the interaction between protein M and IgY. The results showed protein M could be a superior affinity reagent for IgY, scFv as well as IgY Delta Fc, based on pull down and western blot investigations; in addition, it was found that 125 times increase of effective IgY in the elutent was obtained using protein M affinity chromatography column compared with traditional IgY extraction methods. This indicates, the purification strategy of protein M is entirely different to traditional IBPs and the salient purification feature of protein M would be a breakthrough for purifying not only non-mammalian antibodies, but also monoclonal antibodies and engineered antibodies based on variable region. (C) 2016 Elsevier B.V. All rights reserved.

A notable part of the diverse opus of Avgust Pavel is also literary creation. In addition to being a scholar who studied and described the language with the use of scientific principles, his creative personality hid a developing poet who experimented with the expressive and aesthetic potential of language. The poet in him spoke loudest in the third and fifth decade of Pavel's life. In the period of 1907-1917, a Prekmurje magazine published poems in Prekmurian and Hungarian. In 1933, his Hungarian poetry collection Vak Volgy Olena igy zsolozsmazok (I sing the psalms in the arms of a blind valley) was published, followed by the collection Felgyujtott Erdo (Zazgani gozd/Burnt wood) in 1936. In this paper we present Pavel's poetry in the Prekmurje dialect, which is recorded in terms of literary and thematic motifs in the context of older Prekmurje poetry, but has not yet been subject to comprehensive scientific description. Avgust Pavel joined the Prekmurje Catholic religious-cultural circle as a literary colleague, which published poems in the Prekmurje language in its periodicals: Calendar of the Hearts of Jesus (1904-1944), religious monthly St. Mary's Journal (1904-1941) and the weekly Novine (1904-1941). Aside from the poetry of Joseph Basha Miroslav, Pavel's poems represent the literary-aesthetic peak of older, pre-1919 Prekmurje poetry. Pavel's poetic lyricism stems from his personal existential experiences and reflections, while at the same time ingrown in the Prekmurje environment, so that poems about family and community are intertwined with life reflections of poetic meditations. Although many of his poems reference actual persons and events, Pavel's poetry is not adventure poetry, but rather full of experience-based and thoughtful expression of his subjectivity. Considering the addressees and their literary horizon, Avgust Pavel chose traditional poetic images and forms as his lyrical medium. The verse-rhythm organization of poems is based on the principles of accent-based and syllable-accent versification, thus participating in the syllable priming process of the old syllable-based verse which we encounter in Prekmurje poetry as late as the second half of the 19th century. With regard to his profession, Avgust Pavel's predominant poetic voice is the author-scholar, a type which differs from the typical author-priest that dominated older Prekmurje literature.