Anti-CTSW (full length) polyclonal antibody

Proteases are involved in the degradation of the extracellular matrix (ECM), which contributes to the formation of abdominal aortic aneurysm (AAA). To identify new disease targets in addition to the results of previous microarray studies, we performed next-generation sequencing (NGS) of the whole transcriptome of Angiotensin II-treated ApoE(-/-) male mice (n = 4) and control mice (n = 4) to obtain differentially expressed genes (DEGs). Identified DEGs of proteases were analyzed using weighted gene coexpression network analysis (WGCNA). RT-qPCR was conducted to validate the differential expression of selected hub genes. We found that 43 DEGs were correlated with the expression of the protease profile, and most were clustered in immune response module. Among 26 hub genes, we found that Mmp16 and Mmp17 were significantly downregulated in AAA mice, while Ctsa, Ctsc, and Ctsw were upregulated. Our functional annotation analysis of genes coexpressed with the five hub genes indicated that Ctsw and Mmp17 were involved in T cell regulation and Cell adhesion molecule pathway, respectively, and that both were involved in general regulation of the cell cycle and gene expression. Overall, our data suggest that these ectopic genes are potentially crucial to AAA formation and may act as biomarkers for the diagnosis of AAA.

During initial plant development stage, an experiment was conducted to evaluate the balance of salts in the substrate used for the production of 10 hybrids from the cross between `Sunki' mandarin (TSKC) and `Swingle' citrumelo (CTSW), all with potential to be used as rootstock. `Rangpur Santa Cruz' lime, `Sunki Tropical' mandarin and the hybrid LVK (`Volkamer' lemon) x LCR (`Rangpur' lime) - 038 were included as controls, totaling 13 genotypes. Substrate samples were collected in the experiment conducted in a greenhouse at the Federal University of Campina Grande, Campus of Pombal, from December 2015 to June 2016. Two levels of irrigation water salinity were tested, using a 2 x 13 factorial scheme, with 4 replicates. The substrate was a mixture of vermiculite, pine bark and humus (1: 1: 1). For the salinity level of 3 dS m(-1), the substrate is less salinized when cultivated with the hybrids TSKC x CTSW - 044, TSKC x CTSW - 045, TSKC x CTSW - 048, TSKC x CTSW - 049 and `Rangpur Santa Cruz' lime. On the other hand, highest salt concentration was obtained in the substrate cultivated with TSKC x CTSW - 042, TSKC x CTSW - 047, TSKC x CTSW - 048, TSKC x CTSW - 053, TSKC x CTSW - 055 and TSKC x CTSW - 057.