Anti-CLP1 (aa 307-336) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 307-336 of Human HEAB (NP_006822.1, NP_001136069.1)

Conjugate

Application Notes

WB: 1/100 - 1/500.

Alternative Names

CLP1; cleavage and polyadenylation factor I subunit 1; HEAB; PCH10; hClp1; polyribonucleotide 5-hydroxyl-kinase Clp1; ATP/GTP-binding protein; polynucleotide kinase Clp1; polyadenylation factor Clp1; homolog of yeast CFIA subunit Clp1p; pre-mRNA cleavage

Entrez Gene ID

UniProt ID

Antigen Description

Polynucleotide kinase that can phosphorylate the 5-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5-terminus of the tRNA 3-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3-end formation. Also phosphorylates the 5-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Pathway

Cleavage of Growing Transcript in the Termination Region; Processing of Capped Intron-Containing Pre-mRNA; Processing of Intronless Pre-mRNAs; RNA Polymerase II Transcription Termination