Rabbit Anti-Human Cdk8 (N-terminal) Polyclonal antibody

Name: Rabbit Anti-Human Cdk8 (N-terminal) Polyclonal antibody
SKU: DPABH-28762
Rating: 5 (1 reviews)

Rabbit Anti-Human Cdk8 (N-terminal) Polyclonal antibody for WB, IHC, ELISA

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COA

SDS

Datasheet

Key features and details

Target: CDK8
Host: Rabbit
Application: WB, IHC, ELISA
Species Reactivity: Human, Mouse

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse

Immunogen

Synthesized peptide derived from the N-terminal region of human Cdk8.

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-1:2000
IHC: 1:100-1:300
ELISA: 1:10000

General Notes

This product (Catalog No. DPABH-28762) is a rabbit-derived polyclonal antibody raised against human CDK8. This antibody exhibits cross-reactivity with CDK8 from mouse. It has been affinity purified by Protein A. This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
DPABH-28762 is suitable for use in Western blot (WB) (1:500–1:2,000), IHC (1:100–1:300), ELISA (1:10000) applications.
DPABH-28762 is supplied as a liquid formulation in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

CDK8; Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35

Entrez Gene ID

1024

UniProt ID

P49336

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

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References

Genome-wide occupancy profile of mediator and the Srb8-11 module reveals interactions with coding regions

MOLECULAR CELL

Authors: Zhu, XF; Wiren, M; Sinha, I; Rasmussen, NN; Linder, T; Holmberg, S; Ekwall, K; Gustafsson, CM

Abstract

Mediator exists in a free form containing the Med12, Med13, CDK8, and CycC subunits (the Srb8-11 module) and a smaller form, which lacks these four subunits and associates with RNA polymerase II (Pol II), forming a holoenzyme. We use chromatin immunoprecipitation (ChIP) and DNA microarrays to investigate genome-wide localization of Mediator and the Srb8-11 module in fission yeast. Mediator and the Srb8-11 module display similar binding patterns, and interactions with promoters and upstream activating sequences correlate with increased transcription activity. Unexpectedly, Mediator also interacts with the downstream coding region of many genes. These interactions display a negative bias for positions closer to the 5' ends of open reading frames (ORFs) and appear functionally important, because downregulation of transcription in a temperature-sensitive med17 mutant strain correlates with increased Mediator occupancy in the coding region. We propose that Mediator coordinates transcription initiation with transcriptional events in the coding region of eukaryotic genes.

Two conserved modules of Schizosaccharomyces pombe Mediator regulate distinct cellular pathways

NUCLEIC ACIDS RESEARCH

Authors: Linder, Tomas; Rasmussen, Nina N.; Samuelsen, Camilla O.; Chatzidaki, Emmanouella; Baraznenok, Vera; Beve, Jenny; Henriksen, Peter; Gustafsson, Claes M.; Holmberg, Steen

Abstract

Mediator is an evolutionary conserved coregulator complex required for transcription of almost all RNA polymerase II-dependent genes. The Schizosaccharomyces pombe Mediator consists of two dissociable componentsa core complex organized into a head and middle domain as well as the Cdk8 regulatory subcomplex. In this work we describe a functional characterization of the S. pombe Mediator. We report the identification of the S. pombe Med20 head subunit and the isolation of ts alleles of the core head subunit encoding med17. Biochemical analysis of med8(ts), med17(ts), med18, med20 and med27 alleles revealed a stepwise head domain molecular architecture. Phenotypical analysis of Cdk8 and head module alleles including expression profiling classified the Mediator mutant alleles into one of two groups. Cdk8 module mutants flocculate due to overexpression of adhesive cell-surface proteins. Head domain-associated mutants display a hyphal growth phenotype due to defective expression of factors required for cell separation regulated by transcription factor Ace2. Comparison with Saccharomyces cerevisiae Mediator expression data reveals that these functionally distinct modules are conserved between S. pombe and S. cerevisiae.