Recombinant fusion protein of human CDC45 (NP_003495.1).
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:500-1:2000
General Notes
This product (Catalog No. DPABH-18276) is a rabbit-derived polyclonal antibody raised against human CDC45. This antibody exhibits cross-reactivity with CDC45 from mouse and rat. It has been affinity purified by Protein A. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. DPABH-18276 is suitable for use in Western blot (WB) (1:500–1:2,000) application. DPABH-18276 is supplied as a liquid formulation in PBS with 0.02% sodium azide,50% glycerol,pH7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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