Anti-CDC27 monoclonal antibody (DCABH-49)

Mouse anti-Human CDC27 monoclonal antibody for IP, WB, IHC-Fr, IHC-P, ELISA, FC Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2b
Clone
BG4.2
Species Reactivity
Mouse, Rat, Cow, Dog, Human, Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 814-823 of Human Cdc27.
Conjugate
Unconjugated

Applications


Application Notes
WB: 1 - 2 μg/ml; Flow Cyt: 1μg/106 cells;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
CDC27; cell division cycle 27 homolog (S. cerevisiae); cell division cycle 27 , D17S978E, D0S1430E; cell division cycle protein 27 homolog; ANAPC3; anaphase promoting complex subunit 3
Entrez Gene ID
UniProt ID

Product Background


Pathway
APC/C complex, organism-specific biosystem; APC/C complex, conserved biosystem; APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem;

Citations


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References


Shikata, K; Ohta, S; et al. The human homologue of fission yeast cdc27, p66, is a component of active human DNA polymerase delta. JOURNAL OF BIOCHEMISTRY 129:699-708(2001).
Peter, M; Castro, A; et al. The APC is dispensable for first meiotic anaphase in Xenopus oocytes. NATURE CELL BIOLOGY 3:83-87(2001).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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