A water-soluble AIE-active polyvalent glycocluster: design, synthesis and studies on carbohydrate-lectin interactions for visualization of Siglec distributions in living cell membranes
CHEMICAL COMMUNICATIONS
Authors: Liu, Guang-jian; Zhang, Yuan; Zhou, Lingyun; Jia, Li-yan; Jiang, Guohua; Xing, Guo-wen; Wang, Shu
Abstract
In this work, we designed and synthesized an aggregation-induced emission (AIE)-active tetraphenylethene-decorated pseudo-trisialic acid (TPE3S) and validated its high affinity for Siglecs using microscale thermophoresis techniques. TPE3S was a unique binding-on fluorescent trivalent sialocluster which was successfully utilized for the visualization of Siglecs expressed on the surface of mammalian cells.
CD13 expression in B cell malignancies is a hallmark of plasmacytic differentiation
BRITISH JOURNAL OF HAEMATOLOGY
Authors: Raimbault, Anna; Machherndl-Spandl, Sigrid; Itzykson, Raphael; Clauser, Sylvain; Chapuis, Nicolas; Mathis, Stephanie; Lauf, Jeroen; Alary, Anne-Sophie; Burroni, Barbara; Kosmider, Olivier; Fontenay, Michaela; Bene, Marie C.; Durrieu, Francoise; Bettelheim, Peter; Bardet, Valerie
Abstract
The diagnosis of Waldenstrom Macroglobulinaemia (WM)/lymphoplasmacytic lymphoma (LPL) remains one of exclusion because other B-cell lymphoproliferative disorders (B-LPD), such as marginal zone lymphoma (MZL), can fulfil similar criteria, including MYD88 L265P mutation. It has been suggested that expression of the myeloid marker CD13 (also termed ANPEP) is more frequent in LPL than in other B-LPD and has also been described on normal and malignant plasma cells. Here, CD13 expression was tested in a cohort of 1037 B-LPD patients from 3 centres by flow cytometry. The percentage of CD13-expressing cells was found to be variable among B-LPD but significantly higher in WM/LPL (median 31% vs. 0% in non-WM/LPL, P < 0 center dot 001). In multivariate linear regression, CD13 expression remained significantly associated with a diagnosis of WM/LPL (P < 0 center dot 001). A cut-off value of 2% of CD19(+) cells co-expressing CD13 yielded the best diagnostic performance for WM/LPL assertion. This was further improved by association with the presence or absence of IgM paraprotein. Finally, given that previously published transcriptomic data revealed no difference in CD13 (also termed ANPEP) mRNA between normal and pathological B-cells, the hypothesis of some post-transcriptional regulation must be favoured. These results suggest that testing for CD13 expression in routine flow cytometry panels could help to discriminate WM/LPL from other B-LPD.
COA
Publication 
Loading ......
