Recombinant human CCDC102B protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 1-240 aa of BC126448
Conjugate
Unconjugated
Applications
Application Notes
IP: 0.5-4.0 μg for 1.0-3.0 mg of total protein lysate IF/ICC: 1:50-1:500
General Notes
This product (Catalog No. DPABH-21255) is a rabbit-derived polyclonal antibody raised against human CCDC102B, targeting the amino acid region 1–240 of the protein. It has been affinity purified by Protein A. Coiled coil domain containing 102B (CCDC102B) is a new centrosome-linker protein that is required for maintaining centrosome cohesion. Human CCDC102B is an uncharacterized protein containing 513 amino acids (aa); it is predicted to contain one short coiled-coil domain on its N-terminus and two long coiled-coil domains in the middle region and C-terminus, respectively (PMID: 35957886). At the onset of mitosis, along with other centrosome components, CCDC102B is phosphorylated by Nek2A and is disassociated from the centrosome to allow for the centrosome separation (PMID: 30404835). DPABH-21255 is suitable for use in IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IF/ICC (1:50–1:500) applications. DPABH-21255 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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