Anti-CAEV Monoclonal antibody

Name: Anti-CAEV Monoclonal antibody
SKU: DMAB28424
Rating: 5 (1 reviews)

Mouse Anti-CAEV Monoclonal antibody for IP, IF

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COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG1

Species Reactivity

CAEV

Conjugate

Unconjugated

Target

Alternative Names

CAEV; Caprine arthritis encephalitis virus; CAEV ELISA Kit; CaVgp4; putative; NP_040941.1; CAEV ELISA; Caprine arthritis encephalitis virus ELISA Kit; Caprine arthritis encephalitis virus ELISA

Citations

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Cocultivation of caprine arthritis encephalitis virus-infected macrophages with primarygoat synovial cells

Supachart Panneum, Wilairat Chumsing, Theera Rukkwamsuk

Turkish Journal of Veterinary & Animal Sciences2017Read Article

Applications: IF
Reactive species: CAEV
"Abstract: Primary goat synovial cells were developed to be cocultivated with caprine arthritis encephalitis virus (CAEV)-infected monocytes. Two CAEV-seropositive goats and one CAEV-seronegative goat were confirmed for viral particles in the blood using the PCR method. Thereafter, heparinized blood samples from the three goats were collected and isolated for monocytes, which were further cultivated to differentiate into macrophages. Cultivated macrophages of 7-10 days old were cocultivated with the primary goat synovial cells for 7 days. Microscopic examination was performed every 2 days for multinucleated syncytial formation, and immunofluorescence assay was applied using monoclonal antibody against CAEV on day 7 to detect viral particles. Results showed that the primary goat synovial cells cultivated with CAEV-infected macrophages from the two seropositive goats had multinucleated syncytial formation with positive immunofluorescence, while the cocultured macrophages from the seronegative goats showed normal appearance with negative immunofluorescence. The multinucleated syncytial cells were prepared for transmission electron microscope examination and the results indicated that CAEV particles were clearly identified. In conclusion, our developed primary goat synovial cells and the cultivating system were proven to be an appropriate isolation assay for CAEV, which was the first report of cultivation and isolation of CAEV in goats in Thailand."
Article snippet: For immunoffuorescence assay, the mouse anti-CAEV monoclonal antibody(CD Creative Diagnostics), a primary monoclonal antibody that can detect the p28 core polypeptide epitope of the virus at a dilution of 1:100, was applied to the slides to detect the viral particles by confocal immunoffuorescence microscope.

Immunoffuorescence assay indicated the CAEV antigen in goat synovial cells by retained green fuorescence. (Supachart Panneum, <i>et al</i>, 2017)

Figure 1. Immunoffuorescence assay indicated the CAEV antigen in goat synovial cells by retained green fuorescence.

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References

COMPARATIVE FUNCTIONAL-ANALYSIS OF THE VARIOUS LENTIVIRUS LONG TERMINAL REPEATS IN HUMAN COLON-CARCINOMA CELL-LINE (SW480 CELLS) AND FELINE RENAL-CELL LINE (CRFK CELLS)

JOURNAL OF VETERINARY MEDICAL SCIENCE

Authors: MIYAZAWA, T; KAWAGUCHI, Y; KOHMOTO, M; TOMONAGA, K; MIKAMI, T

Abstract

Basal promoter activities of various lentiviral long terminal repeats (LTRs) in a human colon carcinoma cell line (SW480 cells) and a feline renal cell line (CRFK cells) were examined by the chloramphenicol acetyltransferase (CAT) assay using the LTR-CAT reporter plasmids. In SW480 cells, the basal promoter activities induced by LTRs of visna virus, caprine arthritis-encephalitis virus (CAEV), and simian immunodeficiency virus (SIV(AGM)) were moderate, and those induced by LTRs of human immunodeficiency virus (HIV) type 1 (HIV-1) and HIV type 2 (HIV-2) were low. However, the activity induced by the LTR of feline immunodeficiency virus (FIV) was extremely low. In CRFK cells, the basal promoter activities induced by LTRs of visna virus, CAEV and SIV(AGM) were relatively high, and those induced by LTRs of HIV-1, HIV-2 and FIV were moderate. From these data, although the structure of the LTR of FIV is reported to be similar to that of visna virus and CAEV, the function of the LTR of FIV is rather quite different from that of the LTR of these viruses.

MOLECULAR AND GENETIC CHARACTERISTICS OF SMALL RUMINANT LENTIVIRUSES IN SLOVENIA

ACTA VETERINARIA HUNGARICA

Authors: Kuhar, Urska; Barlic-Maganja, Darja; Zadnik, Tomaz; Grom, Joze

Abstract

Small ruminant lentiviruses (SRLV) are spread throughout the world, including Slovenia, where the first evidence of caprine arthritis encephalitis virus (CAEV) infection was found in 1996. This study was conducted to investigate the molecular and genetic characteristics of SRLV infection in Slovenia in order to classify our strains in relation to other known SRLV strains worldwide as well as to establish molecular techniques in concordance with serology. In this study, 340 goats and sheep were tested. Serological examination revealed that 57% of the goats and only 14% of the sheep were seropositive. The results of this study also show that the polymerase chain reaction (PCR) used in this study is less reliable than ELISA, with only 60.6% of the seropositive animals being PCR positive. Thirty-eight nucleotide sequences of the gag region encoding the matrix protein were determined and compared to sequences derived from the GenBank, revealing that Slovenian SRLV strains belong to sequence groups A and B, being maedivisna virus (MVV) and CAEV-like, respectively. In one goat herd, the presence of more than one genotype was confirmed and the majority of goat SRLV sequences were more closely related to MVV than to CAEV prototype strains.