Anti-C7 (aa 145-159) polyclonal antibody

C8 alpha and C9 mediate the membrane attack complex formation and bacterial lysis and are important components in the complement system. The cDNA sequences of the C8 alpha and C9 genes were cloned from Takifugu rubripes. The full-length cDNA of Tr-C8 alpha was 1893 bp and included a 5'-UTR of 69 bp and 3'-UTR of 83 bp. The full-length cDNA of Tr-C9 was 2083 bp and included a 5'-UTR of 72 bp and 3'-UTR of 250 bp. The expression of Tr-C8 alpha and Tr-C9 was detected in newly fertilized eggs of T. rubripes. The expression of these two genes was at a higher level in the liver than in other tissues tested. After lipopolysaccharide (LPS) challenge, the gene expression of Tr-C8 alpha and Tr-C9 increased more significantly in the liver. With these combined results, we further understood how Tr-C8 alpha and Tr-C9 function in the innate immunity of pufferfish. Our findings could deepen the understanding of immune regulation in pufferfish.

Background: To investigate associations between long-term shoulder loading and sagittal spino-pelvic morphology in Chinese farmers from radiology evidences. Methods: We retrospectively analyzed 463 back pain patients who attended outpatient and inpatient departments of two hospitals from January 2016 to December 2018, and who had long, standing lateral X-rays according to inclusion and exclusion criteria. One hundred eighty-four of them were farmers with a long history of heavy shoulder loading for over 20 years in their young age, while others were office workers with no reported long-term shoulder loading history. The following parameters were measured by three researchers independently and then analyzed statistically: thoracic kyphosis (TK), lumbar lordosis (LL), thoracolumbar kyphosis (TLK), T9 sagittal offset (T9SO), T1 sagittal offset (T1SO), sacral slope (SS), pelvic incidence (PI), pelvic tilt (PT), C7 tilt (C7T), spino-pelvic angle (SSA), and sagittal vertical axis (SVA). Results: The "Loading group" included 86 males and 98 females with average age 73.3 (SD 8.3) years, whereas the "Nonloading group" included 126 males and 153 females with average age 63.7 (SD 14.1) years. Age was significantly higher in the loading group (p < 0.001), but gender, height, weight, BMI, and BMD were not significantly different (p > 0.05). The following spino-pelvic parameters were significantly greater (p < 0.05) in the loading group: TK (mean 39.1 degrees vs 32.8 degrees), TLK (25.8 degrees vs 10.1 degrees), and T9SO (12.2 degrees vs 10.1 degrees). Other values were not significantly different between the two groups (p > 0.05). Conclusion: Long-term shoulder loading in youth is a risk factor for pathological thoracic kyphosis especially in the lower thoracic spinal segments when farmers getting older.