Magnesiate-Utilized/Benzyne-Mediated Approach to Indenopyridones from 2-Pyridones: An Attempt To Synthesize the Indenopyridine Core of Haouamine
ORGANIC LETTERS
Authors: Idzik, Tomasz J.; Borzyszkowska-Ledwig, Aleksandra; Struk, Lukasz; Sosnicki, Jacek G.
Abstract
An efficient, short-staged synthesis of cis-fused indeno[2,1-b]- and indeno[1,2-c]pyridin-2-ones, starting from 2-pyridones, using magnesiates of type R3MgLi as nucleophilic and deprotonation agents, mediated by benzyne generated in situ, under optimized conditions, is described. Following the developed protocol, rare C4a-arylsubstituted indeno[2,1-b]pyridones, resembling the core of haouamine, were obtained. The protocol offering the one-pot synthesis directly from 2-pyridone is also described.
Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
INTERNATIONAL BRAZ J UROL
Authors: Ornellas, Paulo; Ornellas, Antonio Augusto; Chinello, Clizia; Gianazza, Erica; Mainini, Veronica; Cazzaniga, Marta; Pereira, Denise Abreu; Sandim, Vanessa; Cypriano, Ana Sheila; Koifman, Leandro; Barbosa da Silva, Paulo Cesar; Alves, Gilda; Magni, Fulvio
Abstract
Purpose: To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods: Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results: A cluster of 2 peptides (A=m/z 1897.22 +/- 9 Da and B=m/z 2021.99 +/- 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions: The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools.
COA
Publication 
Loading ......
