Anti-CD6 monoclonal antibody, clone DD49

In the present work, the effects of dual impeller configurations comprising of Rushton turbine (RT) and Concave-bladed disk turbine (CD-6) on the oxygen transfer profile and fermentation performance of kojic acid production by Aspergillus flavus Link 44-1 was investigated. Batch cultivations of A.flavus Link 44-1 were performed in 2L stirred tank bioreactor. The fermentations were conducted using different dual impeller systems; (1) RT-RT, (2) CD6-CD6, (3) RT-CD6, and (4) CD6-RT (bottom-top impeller). It was perceived that dual CD-6 system was able to improve oxygen transfer rate by about 25-45% over the hybrids of RT and CD-6 and the typically configured dual RT system. While no substantial disparity could be seen on the fungal growth rate by the manipulation of the impeller, high concentration of kojic acid (44.93gL(-1)) was attained with the use of dual CD-6 as the mixer. Efficient agitating system that can facilitate good gas dispersion capability is crucially required in order to counteract the problem of oxygen solubility limitation faced in such viscous fungal fermentation broth. The results from this work suggested the promising capability of dual CD-6 configuration in enhancing productivity of kojic acid fermentation in stirred tank bioreactor. (c) 2014 Curtin University of Technology and John Wiley & Sons, Ltd.

The CD6 glycoprotein is expressed by T lymphocytes and is hypothesized to interact with one or more ligands expressed on antigen presenting cells (APCs). We show that CD6 mediates binding of the transformed CD4+ T cell line Hut 78 to gamma-interferon activated keratinocytes (KCs). A recombinant CD6-Ig fusion protein has been reported to bind to a CD6 ligand ALCAM, but this is the first demonstration that cell-cell adhesion of human T lymphocytes can be CD6 dependent. The known CD6 ligand ALCAM (CD166) is expressed on cultured KCs but does not appear to mediate KC-Hut 78 binding, suggesting the existence of additional CD6 ligands expressed on KCs. In functional studies using autologous KCs as APCs for tetanus toroid specific T cell clones, KCs +/- gamma-interferon are unable to stimulate autologous T cells with recall antigen. Therefore interaction of T cell CD6 with CD6 ligands on KCs does not provide sufficient co-stimulation of primed T cells to support responses to nominal antigen. (C) 1997 Elsevier Science B.V.