General Notes
This product (Catalog No. DPABH-00526) is a rabbit-derived polyclonal antibody raised against human BCL3, targeting the amino acid region 122–360 of the protein. It has been affinity purified by Protein A. BCL3 is a proto-oncogene located on chromosome 19q13.32 that encodes the B-cell lymphoma 3 (Bcl-3) protein, an atypical member of the IκB family of proteins characterized by seven ankyrin repeats and functioning primarily as a transcriptional coactivator for NF-κB homodimers such as p50 and p52. Originally identified through its translocation into the immunoglobulin alpha-locus in some cases of B-cell chronic lymphocytic leukemia (CLL), BCL3 expression forms an autoregulatory loop with NF-κB, influencing nuclear residence and activity of these transcription factors in a context-dependent manner that can either promote or inhibit target gene expression. The protein, with a molecular weight of approximately 47 kDa, features a proline-rich N-terminal domain, central ankyrin repeats for NF-κB binding, and a C-terminal serine- and proline-rich transactivation domain, localizing predominantly to the nucleus while also appearing in cytoplasmic compartments.
DPABH-00526 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-00526 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
COA
Publication 
Loading ......
