Anti-Bat Ig Light Chain Hybridoma [CU2-5G21] (CSC-H0022LN)

This hybridoma produces mAbs (IgG1) against bat Ig light chain

General Information


Immunogen
Immunoglobulin light chain
Isotype
IgG1
Immunological Donor
Mouse
Clone
CU2-5G21
Cell Line Description
The hybridoma was generated by fusion of splenocytes from a BALB/c mouse that had been immunized with big brown bat splenocytes. The resulting hybridomas were plated in methylcellulose and subsequently subcloned in liquid culture. The hybridoma produces a mouse anti-bat Ig Light chain monoclonal antibody. It can bind to B cells and serum immunoglobulin by virtue of specificity for Ig Light Chain.
It has no reactivity against Ig from megachiroptera
Format
Frozen
Growth Properties
Adherent

Culture Method


Complete Growth Medium
RPMI-1640, with 50 uM 2-mercaptoethanol and 2mM glutamine, supplemented with 10% FBS,100 U/ml penicillin and 100 ug/ml streptomycin
Storage
Liquid nitrogen vapor phase
Freezing medium: to FBS, add 10%(v/v) DMSO.

Target


Introduction
The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). A typical antibody is composed of two immunoglobulin (Ig) heavy chains and two Ig light chains.Only one type of light chain is present in a typical antibody, thus the two light chains of an individual antibody are identical.Each light chain is composed of two tandem immunoglobulin domains: one constant (CL) domain and one variable domain (VL) that is important for binding antigen.

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