Anti-BTN3A1 monoclonal antibody, clone CU4.2

Introduction: This study aimed to explore the effect of activated T cells on breast cancer (BC) cells and provide a theoretical basis for the interaction mechanism studies between BC and immune cells. Methods: The microarray dataset GSE73527 was downloaded from the Gene Expression Omnibus database. The common differentially expressed mRNAs (co-DEMs) and the common differentially expressed long non-coding RNAs (co-DElncRNAs) were identified between MDAMB- 231 cells and MCF7 activated human T cells, respectively. The RNA-miRNA-lncRNA (ceRNA) network was constructed. Furthermore, the Kyoto encyclopedia of genes and genomes pathway and the gene ontology function analyses were performed on co-DEMs. The proteinprotein interaction networks and modules were investigated. Results: A total of 639 co-DEMs (such as interleukin-6 [IL6] and signal transducer and activator of transcription 1 [STAT1]) were detected in this study. Defense response to other organisms and herpes simplex infection were the most outstanding function and pathway assembled with co-DEMs, respectively. One protein-protein interaction network and three modules were further constructed. A total of 88 mRNA-miRNA-lncRNA relationships such as BTN3A1-has-mir-20b- 5p-HCP5 were explored in the ceRNA network. Conclusion: Activated T cells may play a crucial role in the defense response to other organism functions and herpes simplex infection pathways by upregulating IL6 and STAT1, which further affected the progression of BC. The BTN3A1-has-miR-20b-5p-HCP5 relationship may be the potential interaction mechanism between BC and immune cells.

We have recently reported the synthesis of the first known phosphoantigen prodrug, and have demonstrated excellent gains in potency through delivery of the phosphoantigen to the intracellular domain of BTN3A1.