Anti-AU1 monoclonal antibody (DMAB7066)

Mouse anti-AU1 monoclonal antibody for IF, WB, IP Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2a
Clone
BV2
Species Reactivity
N/A
Conjugate
Unconjugated

Target


Alternative Names
AU1 epitope; DTYRYI epitope; DTYRYI; AU1

Product Background


Antigen Description
The AU1 tag antibody was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins. U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR. This detected the expression of a 290 kD protein corresponding to the AU1 tagged mutant mTOR proteins in both cell lines. (The mutant mTOR proteins appear beneath a band corresponding to the wild-type (WT) mTOR protein - this was not detected by ab3401 but a different antibody).

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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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