Rabbit Anti-Human ATP1B3 (internal) Polyclonal antibody

Name: Rabbit Anti-Human ATP1B3 (internal) Polyclonal antibody
SKU: DPABH-02356
Rating: 5 (1 reviews)

Rabbit Anti-Human ATP1B3 (internal) Polyclonal antibody for WB

COA

SDS

Datasheet

Key features and details

Target: ATP1B3
Host: Rabbit
Application: WB
Species Reactivity: Human

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Synthesized peptide derived from Internal of human ATP1B3.

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-1:3000

General Notes

This product (Catalog No. DPABH-02356) is a rabbit-derived IgG polyclonal antibody raised against human ATP1B3. It has been affinity purified by protein A. The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2.
DPABH-02356 is suitable for use in Western blot (WB) (1:500–1:3,000) application.
DPABH-02356 is supplied as a liquid formulation in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

AT1B3; ATP1B3; ATPase; Na+/K+ transporting; beta 3 polypeptide

Entrez Gene ID

483

UniProt ID

P54709

Citations

Have you cited DPABH-02356 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

COMPARATIVE IN SILICO ANALYSIS OF ATP1A3 GENE IN MAMMALS

EXPLORATORY ANIMAL AND MEDICAL RESEARCH

Authors: Mishra, Chinmoy; Sahoo, Siddhant Sekhar; Mishra, Sidharth Prasad; Mohanty, Stuti Tanaya; Nayak, Gangadhar; Behera, Kumaresh; Sethy, Kamdev

Abstract

Progress in the field of bioinformatics is useful to understand the global network of genes and their protein products. Genetic variants of the ATP1A3 gene have been suggested to be involved in the salt hypertension and feed intake, all of which may evidently affect heat production, heat loss and water mineral balance. In the present study, comparative analysis of ATP1A3 (ATPase Na+/K+ Transporting Subunit Alpha 3) proteins of different livestock species were carried out using bioinformatics tools. The results of this study indicate that most of physico-chemical properties were almost same in pig and cat. The global network analysis of ATP1A3 gene by, STRING 10 tool speculated its interaction with several other proteins like - ATP1A1, ATP1A2, ATP1A4, ATP1B1, ATP1B3, FXYD2 in different livestock species having high confidence score. The strong interaction was seen between ATP1B1 and ATP1A3 with a high score in pig. Molecular docking between ATP1A3 and ATP1B1 of pig has been carried out. Conserved region was present in all the nine studied different livestock species protein sequences despite speciation due to evolution. The present study will further support to understand the role of associated proteins in various cellular pathways in cattle. This work is also useful for the study of structural and functional analysis of ATP1A3 protein.

Absence of a significant linkage between Na+,K+-ATPase suburmit (ATP1A3 and ATP1B3) genotypes and bipolar affective disorder in the old-order Amish

AMERICAN JOURNAL OF MEDICAL GENETICS

Authors: Philibert, RA; Cheung, D; Welsh, N; Damschroder-Williams, P; Thiel, B; Ginns, EI; Gershenfeld, HK

Abstract

Previous studies provide evidence for a genetic component for susceptibility to bipolar affective disorder (BPAD) in the old-order Amish population. El-Mallakh and Wyatt [1995: Biol Psychiatry 37:235-244] have suggested that the Na+,K+-ATPase may be a candidate gene for BPAD, This study examines the relationship between BPAD in the old-order Amish cohort and the Na+,K+-ATPase alpha1 and beta3 subunit genes (ATP1A3, ATP1B3). A total of 166 sibling pairs were analyzed for linkage via nonparametric methods, Suggestive levels of statistical significance were not reached in any stratification model for affective illness, Overall, the results do not support linkage of bipolar disorder to the Na+,K+-ATPase alpha subunit gene (ATP1A3) and beta subunit gene (ATP1B3) in these old-order Amish families and they show that these Na+,K+-ATPase subunit genes are not major effect genes (greater than or equal to fourfold increased genetic risk of disease) for BPAD in the old-order Amish pedigrees. We cannot exclude other genetic variants of the Na+,K+-ATPase hypothesis for BPAD, whereby other loci may modifying Na+,K+-ATPase activity, (C) 2001 Wiley-Liss, Inc.