Anti-Human ATP1B3 (internal) Polyclonal antibody

Previous studies provide evidence for a genetic component for susceptibility to bipolar affective disorder (BPAD) in the old-order Amish population. El-Mallakh and Wyatt [1995: Biol Psychiatry 37:235-244] have suggested that the Na+,K+-ATPase may be a candidate gene for BPAD, This study examines the relationship between BPAD in the old-order Amish cohort and the Na+,K+-ATPase alpha1 and beta3 subunit genes (ATP1A3, ATP1B3). A total of 166 sibling pairs were analyzed for linkage via nonparametric methods, Suggestive levels of statistical significance were not reached in any stratification model for affective illness, Overall, the results do not support linkage of bipolar disorder to the Na+,K+-ATPase alpha subunit gene (ATP1A3) and beta subunit gene (ATP1B3) in these old-order Amish families and they show that these Na+,K+-ATPase subunit genes are not major effect genes (greater than or equal to fourfold increased genetic risk of disease) for BPAD in the old-order Amish pedigrees. We cannot exclude other genetic variants of the Na+,K+-ATPase hypothesis for BPAD, whereby other loci may modifying Na+,K+-ATPase activity, (C) 2001 Wiley-Liss, Inc.

Progress in the field of bioinformatics is useful to understand the global network of genes and their protein products. Genetic variants of the ATP1A3 gene have been suggested to be involved in the salt hypertension and feed intake, all of which may evidently affect heat production, heat loss and water mineral balance. In the present study, comparative analysis of ATP1A3 (ATPase Na+/K+ Transporting Subunit Alpha 3) proteins of different livestock species were carried out using bioinformatics tools. The results of this study indicate that most of physico-chemical properties were almost same in pig and cat. The global network analysis of ATP1A3 gene by, STRING 10 tool speculated its interaction with several other proteins like - ATP1A1, ATP1A2, ATP1A4, ATP1B1, ATP1B3, FXYD2 in different livestock species having high confidence score. The strong interaction was seen between ATP1B1 and ATP1A3 with a high score in pig. Molecular docking between ATP1A3 and ATP1B1 of pig has been carried out. Conserved region was present in all the nine studied different livestock species protein sequences despite speciation due to evolution. The present study will further support to understand the role of associated proteins in various cellular pathways in cattle. This work is also useful for the study of structural and functional analysis of ATP1A3 protein.