General Notes
This product (Catalog No. DPABH-02590) is a rabbit-derived polyclonal antibody raised against human APPL1, targeting the amino acid region 1–323 of the protein. This antibody exhibits cross-reactivity with Human APPL1 from mouse and rat. It has been affinity purified by Protein A. Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].
DPABH-02590 is suitable for use in Western blot (WB) (1:1,000–1:4,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:20–1:200), IF/ICC (1:50–1:500), flow cytometry (FC) (0.40 μg per 10^6 cells in a 100 μl suspension) applications.
DPABH-02590 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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